Biochemical characterization of Walterinnesia morgani (desert black cobra) venom (Serpentes: Elapidae)
نویسندگان
چکیده
Snake venom contains various bioactive proteins and peptides, of which enzymes make up a significant portion. Desert Black Cobra (Walterinnesia morgani) is venomous snake distributed mainly in the Middle East including southeastern Türkiye. The aim present study to investigate key enzyme activities protein profile W. morgani originating from Sanliurfa province. After determination content, sample was subjected enzymatic activity assays assess phospholipase A2, protease, ʟ-amino acid oxidase, hyaluronidase, 5′-nucleotidase and, phosphodiesterase by spectrophotometry-based method. Protease also assessed gelatin zymography. Additionally, fibrinogenolytic evaluated using fibrinogen zymography SDS-PAGE methods. obtained (both reduced non-reduced) reversed-phase HPLC According results, 11 bands between approximately 12-240 kDa were observed on non-reduced gel while there nine 12-140 gel. Venom found predominantly 25-12 kDa. Proteins separated into at least 19 major minor groups (peaks) analysis. showed all varying levels.
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ژورنال
عنوان ژورنال: Biotech studies
سال: 2023
ISSN: ['2687-3761']
DOI: https://doi.org/10.38042/biotechstudies.1310004